Gene duplication confers enhanced expression of 27-kDa γ-zein for endosperm modification in quality protein maize

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Gene duplication confers enhanced expression of 27-kDa γ-zein for endosperm modification in quality protein maize.

The maize opaque2 (o2) mutant has a high nutritional value but it develops a chalky endosperm that limits its practical use. Genetic selection for o2 modifiers can convert the normally chalky endosperm of the mutant into a hard, vitreous phenotype, yielding what is known as quality protein maize (QPM). Previous studies have shown that enhanced expression of 27-kDa γ-zein in QPM is essential for...

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γ-Zeins are essential for endosperm modification in quality protein maize

Zeins are essential for endosperm modification in quality protein maize" Essential amino acids like lysine and tryptophan are deficient in corn meal because of the abundance of zein storage proteins that lack these amino acids. A natural mutant, opaque 2 (o2) causes reduction of zeins, an increase of nonzein proteins, and as a consequence, a doubling of lysine levels. However, o2's soft inferio...

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Functional Genomics of Maize Endosperm Maturation and Protein Quality

Quality Protein Maize (QPM) is a hard kernel variant of the high-lysine mutant, opaque2. Using γ-irradiation, we created opaque QPM variants to identify opaque-2 modifier genes and to investigate deletion mutagenesis combined with Illumina sequencing as a maize functional genomics tool. A K0326Y-QPM deletion mutant (line 107) was null for the 27and 50-kD γ-zeins and abolished vitreous endosperm...

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Gamma-zeins are essential for endosperm modification in quality protein maize.

Essential amino acids like lysine and tryptophan are deficient in corn meal because of the abundance of zein storage proteins that lack these amino acids. A natural mutant, opaque 2 (o2) causes reduction of zeins, an increase of nonzein proteins, and as a consequence, a doubling of lysine levels. However, o2's soft inferior kernels precluded its commercial use. Breeders subsequently overcame ke...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 2016

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.1601352113